Related Experiment Video
Updated: Jun 10, 2026
In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability
Published on: October 19, 2018
Intestinal permeability assessed by 51Cr-EDTA in rats with CCl4 - induced cirrhosis
Ana Regina L Ramos1, Ursula Matte, Helena Ayako Sueno Goldani
1Experimental Laboratory of Gastroenterology and Hepatology, Hospital de Clínicas de Porto Alegre, Porto Alegre-RS, Brazil. anareginalimaramos@yahoo.com.br
Context:
The straight relationship between cirrhosis and impaired intestinal barrier has not been elucidated yet.
Objectives:
To verify (51)Cr-EDTA-intestinal permeability in rats with CCl(4)-induced cirrhosis and controls.
Method:
Fifty male Wistar rats weighing 150-180 g were separated in three groups: 25 animals received CCl(4) 0.25 mL/kg with olive oil by gavage with 12 g/rat/day food restriction for 10 weeks (CCl(4)-induced cirrhosis); 12 received the same food restriction for 10 weeks (CCl(4)-non exposed). Other 13 rats received indomethacin 15 mg/kg by gavage as positive control of intestinal inflammation.
Results:
The median (25-75 interquartile range) (51)Cr-EDTA-IP values of cirrhotic and CCl(4)-non exposed rats were 0.90% (0.63-1.79) and 0.90% (0.60-1.52) respectively, without significant difference (P = 0.65). Animals from indomethacin group showed (51)Cr-EDTA-IP, median 7.3% (5.1-14.7), significantly higher than cirrhotic and CCl(4)-non exposed rats (P<0.001).
Conclusion:
This study showed the lack of difference between (51)Cr-EDTA-intestinal permeability in rats with and without cirrhosis. Further studies are necessary to better clarify the relationship between intestinal permeability and cirrhosis.
