Human skin penetration of a copper tripeptide in vitro as a function of skin layer
Jurij J Hostynek1, Frank Dreher, Howard I Maibach
1Department of Dermatology, UCSF School of Medicine, San Francisco, CA, USA. jurij.hostynek65@gmail.com
Objective And Design:
Skin retention and penetration by copper applied as glycyl-L-histidyl-L-lysine cuprate diacetate was evaluated in vitro in order to assess its potential for its transdermal delivery as an anti-inflammatory agent.
Materials And Methods:
Flow-through diffusion cells with 1 cm(2) exposure area were used under infinite dose conditions. 0.68% aq. copper tripeptide as permeant was applied on isolated stratum corneum, heat-separated epidermis and dermatomed skin and receptor fluid collected over 48 h in 4 h intervals using inductively coupled plasma mass spectrometry to analyze for copper in tissues and receptor fluid.
Results:
The permeability coefficient of the compound through dermatomed skin was 2.43 ± 0.51 × 10(-4) cm/h; 136.2 ± 17.5 μg/cm(2) copper permeated 1 cm(2) of that tissue over 48 h, while 97 ± 6.6 μg/cm(2) were retained as depot.
Conclusions:
Copper as tripeptide was delivered in potentially therapeutically effective amounts for inflammatory disease.
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