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Cell-based assays--Informa Life Sciences' Fifth Annual Conference--Label-free cell-based assays and high-content

Fabio Gasparri1

  • 1Nerviano Medical Sciences Srl, Milan, Italy. fabio.gasparri@nervianoms.com

Idrugs : the Investigational Drugs Journal
|August 20, 2010
PubMed

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Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...