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Optimization, Design and Avoiding Pitfalls in Manual Multiplex Fluorescent Immunohistochemistry
09:15

Optimization, Design and Avoiding Pitfalls in Manual Multiplex Fluorescent Immunohistochemistry

Published on: July 26, 2019

Publication of optimized multicolor immunofluorescence panels

Yolanda Mahnke1, Pratip Chattopadhyay, Mario Roederer

  • 1Immunotechnology Section, Vaccine Research Center, NIH, Bethesda, Maryland, USA.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|August 20, 2010
PubMed
Summary

No abstract available in PubMed .

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Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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