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Updated: Dec 23, 2025

Identification of Mycobacterium Species by DNA Microarray Chip Method
Published on: June 24, 2025
Fewer essential genes in mycoplasmas than previous studies suggest
Kevin Dybvig1, Ping Lao, David S Jordan
1Department of Genetics, University of Alabama at Birmingham, Birmingham, AL, USA. dybvig@uab.edu
Abstract:
Here, we describe mutants of Mycoplasma pulmonis that were obtained using a minitransposon, Tn4001TF1, which actively transposes but is then unable to undergo subsequent excision events. Using Tn4001TF1, we disrupted 39 genes previously thought to be essential for growth. Thus, the number of genes required for growth has been overestimated. This study also revealed evidence of gene duplications in M. pulmonis and identified chromosome segregation proteins that are dispensable in mycoplasmas but essential in Bacillus subtilis.
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