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Ultrastructure and x-ray microanalysis of siderosome
Archives of Dermatological Research
|April 7, 1978
Summary
This study analyzed hemosiderotic histiocytoma skin lesions, finding iron-rich particles within siderosomes. Increased iron content correlated with particle size and electron density, confirming iron accumulation in these cells.
Area of Science:
- Dermatopathology
- Cell Biology
- Biochemistry
Background:
- Hemosiderotic histiocytoma is a skin lesion characterized by iron deposition.
- The precise nature and localization of iron within these lesions require detailed investigation.
Purpose of the Study:
- To ultrastructurally examine iron-containing particles in hemosiderotic histiocytoma.
- To quantitatively analyze the iron content within siderosomes using energy-dispersive electron probe X-ray microanalysis.
Main Methods:
- Comparative ultrastructural examination of skin lesions.
- Energy-dispersive electron probe X-ray microanalysis (EDX) of siderosomes.
- Qualitative and quantitative spectral analysis for iron identification and quantification.
Main Results:
- Characteristic X-ray energy emitted by iron was detected in siderosomes.
- Adjacent cytoplasm showed minimal iron peaks.
- Iron counts intensity in siderosomes increased proportionally with inclusion amount and electron opacity.
Conclusions:
- Siderosomes in hemosiderotic histiocytoma are confirmed to contain iron-rich fine particles.
- The quantitative analysis supports a direct correlation between iron accumulation and siderosome characteristics.
- Further investigation into the chemical nature of inclusions and lipid presence in cytoplasm is warranted.