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Related Experiment Video

Updated: Jun 10, 2026

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Published on: September 1, 2015

Adenosine regulates Sertoli cell function by activating AMPK.

M N Galardo¹, M F Riera, E H Pellizzari

¹Centro de Investigaciones Endocrinológicas (CEDIE-CONICET), Hospital de Niños Ricardo Gutiérrez, Gallo 1330, C1425EDF Buenos Aires, Argentina.

Molecular and Cellular Endocrinology

|August 21, 2010

Summary

This summary is machine-generated.

Adenosine enhances Sertoli cell function by activating AMPK, promoting lactate production and maintaining cell junctions. This supports a healthy microenvironment crucial for successful spermatogenesis.

Related Experiment Videos

Last Updated: Jun 10, 2026

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Published on: September 1, 2015

Area of Science:

Reproductive Biology
Cell Physiology
Molecular Endocrinology

Background:

Sertoli cells are crucial for spermatogenesis, requiring a stable microenvironment.
Adenosine's role in Sertoli cell function is not fully understood, particularly its non-purinergic receptor-mediated effects.

Purpose of the Study:

To investigate the distinct effects of adenosine on Sertoli cell physiology compared to A(1) purinergic receptor activation.
To elucidate the signaling pathways involved in adenosine's actions on Sertoli cells.

Main Methods:

Comparison of adenosine and N(6)-cyclohexyladenosine (CHA) effects on Sertoli cells.
Measurement of lactate production, glucose uptake, and gene/protein expression (GLUT1, LDHA, MCT4, ZO-1).
Assessment of AMP-activated protein kinase (AMPK) activation and functional role using inhibitors.

Main Results:

Adenosine, but not CHA, increased lactate production, glucose uptake, and GLUT1, LDHA, MCT4 mRNA levels.
Adenosine stabilized ZO-1 protein at the cell membrane, unlike CHA.
Adenosine activated AMPK, which was essential for lactate production and ZO-1 stabilization.

Conclusions:

Adenosine exerts effects on Sertoli cells beyond A(1) purinergic receptor activation, involving AMPK.
Adenosine-activated AMPK promotes lactate supply to germ cells.
These actions contribute to maintaining junctional complex integrity and an optimal microenvironment for spermatogenesis.