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Recombination between Sindbis virus RNAs.

B G Weiss1, S Schlesinger

  • 1Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110-1093.

Journal of Virology
|August 1, 1991
PubMed
Summary
This summary is machine-generated.

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Sindbis virus RNA recombination was demonstrated using cloned cDNAs, producing infectious recombinant RNAs with sequence insertions and deletions. Some recombinants evolved toward wild-type RNA structure.

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Sindbis virus has a positive-strand RNA genome (49S RNA) divided into nonstructural and structural protein coding domains.
  • Structural proteins are translated from a 26S subgenomic RNA transcribed from an internal promoter.

Purpose of the Study:

  • To demonstrate RNA recombination between Sindbis virus RNAs in cultured cells.
  • To characterize the genetic makeup and functional properties of resulting recombinant RNAs.

Main Methods:

  • Utilized Sindbis virus RNAs transcribed from cloned cDNAs for recombination experiments.
  • Sequenced recombinant RNAs, particularly in the junction region between nonstructural and structural genes.

Main Results:

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  • Infectious Sindbis virus recombinants were generated from various combinations of altered parental RNAs.
  • Most recombinant RNAs were larger than wild-type, containing sequence insertions and sometimes deletions or rearrangements.
  • Recombination frequently occurred at the junction between nonstructural and structural genes.
  • Functional subgenomic RNA promoters were retained in recombinant RNAs.
  • Recombinants with large insertions showed evolutionary trends towards wild-type RNA.

    • Conclusions:

    • Sindbis virus RNA recombination is feasible in cultured cells using in vitro transcribed RNAs.
    • Recombination can lead to novel genetic combinations and functional RNA molecules.
    • The study provides insights into viral genome evolution and the mechanisms of RNA recombination.