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Updated: Jun 10, 2026

Mitochondrial Ca2+ Retention Capacity Assay and Ca2+-triggered Mitochondrial Swelling Assay
Published on: May 1, 2018
Reduced capacity of Ca²+ retention in liver as compared to kidney mitochondria. ADP requirement
Cecilia Zazueta1, Noemí García, Eduardo Martínez-Abundis
1Departamento de Bioquímica Instituto Nacional de Cardiología, Ignacio Chávez, Juan Badiano # 1, Tlalpan, México DF014080, México.
Abstract:
Ca²+ loading in mitochondria promotes the opening of a non-selective transmembrane pathway. Permeability transition is also associated with the interaction of cyclophilin D at the internal surface of the non-specific transmembrane pore. This interaction is circumvented by cyclosporin A and ADP. Our results show that, in the absence of ADP, liver mitochondria were unable to retain Ca²+, they underwent a fast and large amplitude swelling, as well as a rapid collapse of the transmembrane potential. In contrast, in the absence of ADP, kidney mitochondria retained Ca²+, swelling did not occur, and the collapse of the membrane potential was delayed. Ca²+ efflux was reversed by the addition of ADP and cyclosporin A. Our findings indicate that the differences between liver and kidney mitochondria are due to the low association of cyclophilin D to the ADP/ATP carrier found in kidney mitochondria as compared to liver mitochondria.
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