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[Effects of 2,2',4,4'-tetrabrominated diphenyl ethers on the TRalpha1 and TRbeta1 expression in liver in C57BL/6
Zaoling Liu1, Jiwen Liu, Jianqing Zhang
1School of Public Health of Xinjiang Medicial University, Urumchi 830054, China. 80858710173@sina.com
Objective:
To study the effect of 2,2',4,4'-tetrabrominated diphenyl ethers (BDE-47) on the expression change of TRalpha1, TRbeta1 at both mRNA and protein levels.
Methods:
C57BL/6 mice were divided randomly into 4 groups and administrated with corn oil, 0.5, 5 and 50 mg/kg/BW BDE-47 for 4 days through intraperitoneal injection respectively. GAPDH selected as internal standard, real time quantitative RT-PCR and Western blot were employed to detect the mRNA levels and protein of TRalpha1 and TRbeta1 in livers.
Results:
Compared with the control group, the mRNA levels of TRalpha1 and TRbeta1 were up-regulated in the medium and high BDE-47 dose groups (P < 0.05). The protein level of TRalpha1 significantly up-regulated while TRbeta1 was significantly down-regulated.
Conclusion:
BDE-47 could change the expression of TRalpha1 and TRbeta1 in the mRNA and protein levels in liver.
Insights
2,2′,4,4′-tetrabrominated diphenyl ether (BDE-47) exposure altered thyroid hormone receptor (TR) expression in mice livers. BDE-47 affected both mRNA and protein levels of TRalpha1 and TRbeta1.
Area of Science:
- Environmental toxicology
- Molecular endocrinology
Context:
- Flame retardants like BDE-47 are persistent environmental pollutants.
- Thyroid hormone receptors (TRalpha1 and TRbeta1) are crucial for regulating metabolism and development.
Purpose:
- To investigate the impact of BDE-47 on TRalpha1 and TRbeta1 gene and protein expression in mouse liver.
- To determine dose-dependent effects of BDE-47 exposure.
Summary:
- Mice exposed to BDE-47 showed increased mRNA levels for both TRalpha1 and TRbeta1 at medium and high doses.
- BDE-47 exposure led to significantly increased TRalpha1 protein but decreased TRbeta1 protein levels.
Impact:
- BDE-47 exposure disrupts normal thyroid hormone receptor signaling pathways.
- Findings highlight potential endocrine-disrupting effects of BDE-47 relevant to human health.
