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[Effects of Rap2b gene on foci formation and wound-healing of NIH3T3 cells]
Qiao Zhang1, Liju Duan, Honghui Xu
1Department of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou 450001, China. zhangqiao@zzu.edu.cn
Objective:
To explore the effects on foci formation and wound-healing of NIH3T3 cells, and to provid the experimental evidence of its function.
Methods:
DNA from human lung cells was extracted and amplification of Rap2b gene was done by PCR. Eukaryotic expression vector pcDNA3. 1-Rap2b was constructed and was stable transfected into NIH3T3 cell followed with foci formation assay and wound-healing assay.
Results:
The number of the foci formation of NIH3T3 cell transfected by eukaryotic expression vector pcDNA3. 1-Rap2b was increased remarkably in foci formation assay (P < 0.01) and NIH3T3 cells transfected by eukaryotic expression vector pcDNA3. 1-Rap2b were quickly heal up in wound-healing assay.
Conclusion:
The extrinsic expression of Rap2b could transform NIH3T3 cell using foci formation assay and wound-healing assay. Rap2b gene might play an oncogenic role in tumorigenesis.
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