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Published on: August 9, 2024
[Effect of polypeptide from Chlamys farreri on ultraviolet A-induced Fas and c-fos expression in HaCaT cells]
Jinlian Li1, Na Li, Xiaoqing Dong
1Binzhou Medical College, Binzhou 256603, China. ljl813@sina.com
Objective:
To observe the effect of Polypeptide from Chlamys farreri (PCF) on expression levels of Fas and c-fos in HaCaT cells after ultraviolet A (UVA) radiation, and investigate the molecular mechanism of PCF protecting against UVA-induced damage in HaCaT cells.
Methods:
HaCaT cells were divided into five groups: control group, UVA model group, 5.69 mmol/L PCF group, 2.84 mmol/L PCF group, and 1.42 mmol/L PCF group. Expression levels of Fas and c-fos mRNA was assayed by PCR. Western blot analysis was used to determine the protein levels of Fas and c-fos.
Results:
After UVA exposure, Fas and c-fos expression increased significantly (P < 0.01). 1.42 - 5.69 mmol/L PCF inhibited UVA-induced mRNA and protein expressions of Fas and c-fos in HaCaT cells in dose-dependent manner (P < 0.05).
Conclusion:
PCF could attenuate UVA-induced Fas and c-fos expression, which may attribute to its protective effect against UVA-induced damage in HaCaT cells.
