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Related Experiment Video

Updated: Jun 10, 2026

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
10:15

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers

Published on: July 22, 2015

Using microcantilevers to study the interactions of lipid bilayers with solid surfaces.

Kai-Wei Liu1, Sibani Lisa Biswal

  • 1Department of Chemical and Biomolecular Engineering, Rice University, Houston, Texas 77005, USA.

Analytical Chemistry
|August 24, 2010
PubMed
Summary

Microcantilever beams measure surface free energy changes in phospholipid bilayers. Cholesterol addition decreases lipid bilayer affinity for solid surfaces, revealing new insights into supported lipid membranes.

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Area of Science:

  • Biophysics
  • Materials Science
  • Surface Chemistry

Background:

  • Microcantilever beams are ultrasensitive tools for measuring surface free energy changes.
  • These changes are induced by thin film adsorption onto a substrate.
  • Understanding solid surface and phospholipid bilayer interactions is crucial for biomembrane research.

Purpose of the Study:

  • To utilize microcantilever beams to probe interactions between solid surfaces and phospholipid bilayers.
  • To investigate how electrostatic and intermolecular forces influence these interactions.
  • To measure adsorption free energy of lipid bilayers and the effect of cholesterol.

Main Methods:

  • Employing free-standing microcantilever beams to detect surface free energy variations.

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Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
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Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

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Last Updated: Jun 10, 2026

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
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Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers

Published on: July 22, 2015

Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection
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Nanomechanics of Drug-target Interactions and Antibacterial Resistance Detection

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Published on: August 3, 2021

  • Relating cantilever deflection to surface free energy changes in supported lipid bilayers (SLBs).
  • Modifying SLB surface charge density and observing the impact on cantilever deflection.

    • Main Results:

    • Cantilever deflection correlated with electrostatic and intermolecular interactions between the bilayer and substrate.
    • Increased cationic lipid ratio in SLBs led to greater cantilever deflection.
    • Cholesterol incorporation into lipid bilayers reduced their affinity for the solid surface.

    Conclusions:

    • Microcantilevers provide a novel technique for probing SLB adsorption free energy.
    • The study highlights the influence of lipid composition and cholesterol on bilayer-surface interactions.
    • Findings offer insights into the forces governing supported lipid membrane structure.