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Related Experiment Videos

Low access resistance perforated patch recordings using amphotericin B.

J Rae1, K Cooper, P Gates

  • 1Department of Physiology and Biophysics, Mayo Foundation, Rochester, MN 55905.

Journal of Neuroscience Methods
|March 1, 1991
PubMed
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New perforated patch techniques using amphotericin B significantly improve electrophysiological measurements. These methods achieve low access resistances, enhancing whole-cell and single-channel current recordings, and resting voltage measurements.

Area of Science:

  • Electrophysiology
  • Cell Biology
  • Biophysics

Background:

  • Traditional electrophysiological techniques can be limited by high access resistance.
  • Achieving stable and reliable measurements is crucial for understanding cellular function.

Purpose of the Study:

  • To describe experimental procedures for creating perforated patches using amphotericin B.
  • To demonstrate the utility of these perforated patches for various electrophysiological recordings.

Main Methods:

  • Perforated patch-clamp technique utilizing amphotericin B.
  • Use of blunt electrode tips to reduce access resistance.
  • Application in whole-cell and single-channel current measurements.
  • Measurement of resting voltage in epithelial monolayers.

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Main Results:

  • Achieved access resistances below 10 MΩ in 13 cellular preparations.
  • Attained access resistances of 3-4 MΩ with blunt electrode tips.
  • Successfully measured whole-cell currents, single-channel currents (outside-out patch), and resting voltage.

Conclusions:

  • The developed perforated patch methods offer increased versatility and quality for electrophysiological studies.
  • These techniques are valuable for a wide range of electrophysiological applications.
  • Improved access resistance facilitates more accurate cellular electrical property measurements.