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Updated: Jun 10, 2026
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Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation
Published on: September 29, 2019
Purification and partial characterization of canine S100A12
Romy M Heilmann1, Jan S Suchodolski, Jörg M Steiner
1Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843-4474, USA. rheilmann@cvm.tamu.edu
Biochimie
|August 24, 2010
Summary
Researchers successfully purified canine S100A12, a protein linked to inflammation, from dog blood. This breakthrough paves the way for developing diagnostic tests for canine gastrointestinal inflammation.
Area of Science:
- Biochemistry
- Veterinary Medicine
- Immunology
Background:
- Canine S100A12 (cS100A12) is a calcium-binding protein found in neutrophils and monocytes.
- S100A12's interaction with RAGE suggests a role in inflammation, and it's a marker for gastrointestinal inflammation in humans.
- Purification and immunoassays for S100A12 are limited, with only human, porcine, bovine, and rabbit proteins purified to date.
Purpose of the Study:
- To develop a purification protocol for canine S100A12 (cS100A12).
- To partially characterize cS100A12.
- To lay the groundwork for an immunologic method to detect and quantify cS100A12 in canine samples.
Main Methods:
- Isolation of leukocytes from canine whole blood via dextran sedimentation.
- Extraction of cS100A12 from the cytosol fraction of canine leukocytes.
- Purification using ammonium sulfate precipitation, hydrophobic interaction chromatography, and ion-exchange chromatography.
Main Results:
- Successful purification of cS100A12 from canine whole blood.
- Estimated molecular mass of 10,379.5 Da and an isoelectric point of 6.0.
- 100% N-terminal sequence identity with predicted canine S100A12 and 78.6% homology with other species' S100A12.
Conclusions:
- Canine S100A12 can be effectively purified using the developed methods.
- The characterization provides essential data for future immunoassay development.
- This research enables the potential for diagnosing canine gastrointestinal inflammation.
