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A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces
Published on: March 1, 2017
High density gene expression microarrays and gene ontology analysis for identifying processes in implanted tissue
Gerwen Lammers1, Christian Gilissen, Suzan T M Nillesen
1Department of Biochemistry 280, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
Biomaterials
|August 24, 2010
Summary
High-density gene expression microarrays and gene ontology analysis offer a fast, unbiased method for evaluating tissue-engineered skin constructs in vivo. This approach complements traditional histology, identifying biological processes during wound healing.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Molecular Biology
Background:
- In vivo assessment of tissue-engineered constructs typically relies on conventional methods like histology.
- A need exists for faster, more objective evaluation tools for engineered tissues.
Purpose of the Study:
- To employ high-density gene expression microarrays and gene ontology (GO) analysis as a read-out tool.
- To identify biological processes following implantation of an acellular collagen-based skin construct in a rat model.
- To compare these findings with natural wound healing processes.
Main Methods:
- Utilized high-density gene expression microarrays for global gene expression profiling.
- Applied gene ontology (GO) analysis via the DAVID program to identify biological pathways.
- Performed conventional (immuno)histology for validation of identified biological processes.
- Detailed the step-by-step process from RNA isolation to biological interpretation.
Main Results:
- Microarray and GO analysis identified specific up/downregulated biological processes post-implantation.
- Results were compared to control groups undergoing natural wound healing.
- Conventional histology corroborated the biological processes identified by microarray analysis.
Conclusions:
- Microarray analysis, coupled with GO, presents a valuable, rapid, and unbiased method for evaluating the in vivo performance of tissue-engineered constructs.
- This molecular approach complements traditional histological assessments.
- Further development is needed for specific GO-terms and rat genome annotation.
