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Optimizing the concentration of hydroxyethylstarch in a novel intestinal-specific preservation solution
Matthew S Kokotilo1, Jodi Carter, Aducio Thiesen
1Department of Surgery, University of Alberta, Edmonton, Alberta, Canada.
Cryobiology
|August 24, 2010
Summary
Hydroxyethylstarch (HES) is essential for intestinal preservation solutions. A 5% concentration of HES in a nutrient-rich solution optimally supports energetics and reduces oxidative stress during cold storage.
Area of Science:
- Transplantation research
- Organ preservation science
- Surgical innovation
Background:
- Nutrient-rich solutions enhance energy production and control oxidative stress in static cold storage of intestines.
- The role of oncotic agents, specifically hydroxyethylstarch (HES), in intraluminal preservation solutions was previously unevaluated.
Purpose of the Study:
- To investigate the effectiveness and necessity of hydroxyethylstarch (HES) in an intraluminal preservation solution for intestinal static cold storage.
- To determine the optimal concentration of HES for maintaining intestinal viability during cold storage.
Main Methods:
- Rat intestines were flushed with University of Wisconsin solution and then with an intraluminal preservation solution containing 0%, 2.5%, 5%, or 10% HES.
- Energetics, oxidative stress markers (malondialdehyde), and morphology were assessed over a 24-hour cold storage period.
Main Results:
- The 5% HES solution demonstrated superior energetic status (ATP and total adenylates) compared to all other groups.
- Reduced oxidative stress was observed in groups with 5% and 10% HES.
- Intestines stored with 5% HES showed significantly better morphology after 12 hours compared to controls.
Conclusions:
- Oncotic support is a fundamental requirement for intraluminal preservation solutions during static organ storage.
- A 5% concentration of hydroxyethylstarch (HES) is optimal for preserving intestinal viability.
