Related Experiment Video
Updated: Jun 10, 2026

Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents
Published on: May 16, 2022
Epidermal growth factor regulates PAI-1 expression via activation of the transcription factor Elk-1
Paulina Wyrzykowska1, Krystyna Stalińska, Mateusz Wawro
1Department of Cell Biochemistry, Jagiellonian University, Poland.
Abstract:
PAI-1 (plasminogen activator inhibitor-1) in breast cancer cells is involved in tumour development and metastasis of breast cancer cells. PAI-1 function and the regulation of its expression have been precisely investigated. Here we report that EGF, which promotes breast cancer tumour growth and survival, rapidly induces PAI-1 expression in the breast adenocarcinoma cell line MCF-7 through the activation of the transcription factor Elk-1. We have found that the PAI-1 promoter fragment (-140 to +173) containing the Ets consensus binding site is activated by Elk-1. Chromatin immunoprecipitation analysis confirms in vivo binding of Elk-1 to the PAI-1 promoter and demonstrates that Elk-1 phosphorylation on the Ets binding site is EGF-dependent.
More Related Videos
Related Concept Videos
Mitogens and the Cell Cycle
TGF - β Signaling Pathway
MAPK Signaling Cascades
Regulation of Angiogenesis and Blood Supply
NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Cell Specific Gene Expression

