Related Experiment Video
Updated: Jun 10, 2026
Whole Blood Assay with Dual Co-Stimulation for Antigen-Specific Analysis of Host Immunity to Fungal and Viral Pathogens
Published on: September 20, 2024
Differences in sensitivity to HMME-mediated photodynamic therapy between EBV+ C666-1 and EBV- CNE2 cells
Buhong Li1, Zheng Chen, Lina Liu
1Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fujian, Fuzhou 350007, China. bhli@fjnu.edu.cn
Objectives:
The aim of this study was to compare the sensitivity of hematoporphyrin monomethyl ether (HMME)-mediated PDT in the Epstein-Barr virus (EBV) positive and negative human nasopharyngeal carcinoma (NPC) cells.
Methods And Materials:
The intracellular uptake of HMME in C666-1 (EBV+) and CNE2 (EBV-) cells was quantitatively determined after incubating with different concentration of HMME. The subcellular localization of HMME was examined under a laser scanning fluorescence microscopy. The survival rate of C666-1 and CNE2 cells after HMME PDT was evaluated with clonogenic assay.
Results:
The EBV- CNE2 cells showed higher intracellular uptake of HMME and lower survival rate than EBV+ C666-1 cells. There was no significant difference in terms of subcellular localization of HMME between C666-1 and CNE2 cells.
Conclusion:
The sensitivity of EBV- CNE2 cells to HMME-mediated PDT was significantly higher than that of EBV+ C666-1 cells. The intracellular uptake of HMME plays a key role in determining the PDT sensitivity.
