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Related Experiment Video

Updated: Jun 10, 2026

Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli
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Highly sensitive thermal detection of thrombin using aptamer-functionalized phase change nanoparticles.

Chaoming Wang1, Mainul Hossain, Liyuan Ma

  • 1NanoScience Technology Center, Department of Mechanical, Materials, and Aerospace Engineering, School of Electrical Engineering and Computer Science, University of Central Florida, Orlando, FL 32826, United States.

Biosensors & Bioelectronics
|August 24, 2010
PubMed
Summary

This study introduces a new thermal biosensing method using RNA aptamer nanoparticles for sensitive thrombin detection. This approach offers a selective, low-cost way to detect thrombin, even in complex samples like serum.

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Published on: September 25, 2016

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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

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Published on: October 6, 2022

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Biosensing

Background:

  • Thrombin detection is crucial for diagnosing and monitoring various medical conditions.
  • Existing detection methods often face limitations in sensitivity, selectivity, or cost.

Purpose of the Study:

  • To develop a novel, highly sensitive, and selective thermal biosensing technique for thrombin detection.
  • To utilize RNA aptamer-functionalized phase change nanoparticles as thermal probes.

Main Methods:

  • RNA aptamer-functionalized phase change nanoparticles were used as thermal probes.
  • Nanoparticles formed sandwiched complexes with thrombin on an aptamer-modified substrate.
  • Thermal signals (melting peaks) were analyzed during temperature scans to quantify thrombin.

Main Results:

  • A detection sensitivity of 22 nM was achieved on flat aluminum surfaces.
  • Sensitivity was enhanced fourfold using high-surface-area silicon nanopillar substrates.
  • The method demonstrated immunity to colored species and direct applicability to serum samples.

Conclusions:

  • The developed thermal biosensing technique offers a highly sensitive and selective method for thrombin detection.
  • Combining aptamer specificity with nanostructure surface area provides a low-cost detection solution.
  • This method shows promise for direct thrombin detection in biological samples like serum.