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Association of Campylobacter jejuni Cj0859c gene (fspA) variants with different C. jejuni multilocus sequence types

C P A de Haan1, R Kivistö, M L Hänninen

  • 1Department of Food Hygiene and Environmental Health, P.O. Box 66, FI-00014 University of Helsinki, Helsinki, Finland. astrid.dehaan@helsinki.fi

Applied and Environmental Microbiology
|August 24, 2010
PubMed

Insights

Campylobacter jejuni fspA1 and fspA2 variants were linked to specific hosts and multilocus sequence typing (MLST) types. The fspA1 variant was more prevalent in human and poultry strains, while fspA2 showed greater sequence diversity.

Area of Science:

  • Microbiology
  • Genetics
  • Veterinary Science

Background:

  • Campylobacter jejuni is a leading cause of bacterial gastroenteritis globally.
  • Genetic diversity within C. jejuni strains contributes to variations in host adaptation and pathogenicity.
  • The Cj0859c gene encodes a surface protein with potential roles in host-pathogen interactions.

Purpose of the Study:

  • To investigate the distribution and genetic variation of Cj0859c variants (fspA1 and fspA2) in Campylobacter jejuni strains.
  • To determine associations between specific Cj0859c variants, host origin (human, poultry, bovine), and genetic relatedness (MLST types).

Main Methods:

  • Genomic DNA extraction from 669 Campylobacter jejuni isolates.
  • PCR amplification and sequencing of the Cj0859c gene.
  • Multilocus sequence typing (MLST) for strain characterization.
  • Statistical analysis to assess variant frequency and associations.

Main Results:

  • Cj0859c variants fspA1 and fspA2 were detected in human, poultry, and bovine C. jejuni strains.
  • The fspA1 variant was significantly more prevalent (P < 0.001) than fspA2 in human and poultry isolates.
  • fspA1 and fspA2 variants showed distinct associations with specific host origins and MLST types.
  • Amino acid sequences of FspA2 were more diverse and frequently truncated compared to FspA1.

Conclusions:

  • The distribution of Cj0859c variants is host-associated in Campylobacter jejuni.
  • Genetic variations in Cj0859c may influence host specificity and adaptation.
  • Further research is needed to understand the functional implications of fspA1 and fspA2 diversity in C. jejuni pathogenesis.

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