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Improved detection of HIV-1 envelope sequences using optimized PCR and inosine-substituted primers
S Cassol1, T Salas, N Lapointe
1Federal Centre for AIDS, Health and Welfare, Ottawa, Canada.
Molecular and Cellular Probes
|April 1, 1991
Abstract:
We describe inosine-substituted 'consensus' primers for the detection of HIV-1 envelope sequences by the polymerase chain reaction. The primers, modifications of SK68 and SK69, are highly specific (100%) and sensitive (greater than 94%) and they prevent false-negative results due to variation in the HIV-1 genome. Consensus primers are needed to ensure the detection of most, if not all, variants of HIV-1 including American, African and newly emerging strains.
