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Related Experiment Video

Updated: Jun 10, 2026

DNA Stable-Isotope Probing (DNA-SIP)

DNA Stable-Isotope Probing (DNA-SIP)

Published on: August 2, 2010

DNA stable-isotope probing (DNA-SIP).

Eric A Dunford¹, Josh D Neufeld

¹Department of Biology, University of Waterloo.

Journal of Visualized Experiments : Jove

|August 24, 2010

Summary

DNA stable-isotope probing (DNA-SIP) identifies microorganisms by tracking nutrient assimilation into biomass. This method aids in assigning metabolic functions within diverse environmental communities.

Area of Science:

Microbiology
Environmental Science
Molecular Biology

Background:

DNA stable-isotope probing (DNA-SIP) is a cultivation-independent technique used to identify active microorganisms.
It assigns metabolic functions to diverse microbial communities in various environments by tracking nutrient assimilation.
Understanding microbial roles is crucial for environmental and ecological studies.

Purpose of the Study:

To provide a detailed video protocol for DNA-SIP, focusing on density gradient ultracentrifugation.
To visually demonstrate the steps involved in gradient fractionation and labeled DNA recovery.
To offer practical guidance, including sample data and critical tips, for successful DNA-SIP analysis.

Main Methods:

Incubation of environmental samples with stable-isotope labeled compounds.

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Published on: November 13, 2013

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Last Updated: Jun 10, 2026

DNA Stable-Isotope Probing (DNA-SIP)

DNA Stable-Isotope Probing (DNA-SIP)

Published on: August 2, 2010

Identification of Metabolically Active Bacteria in the Gut of the Generalist Spodoptera littoralis via DNA Stable Isotope Probing Using 13C-Glucose

Identification of Metabolically Active Bacteria in the Gut of the Generalist Spodoptera littoralis via DNA Stable Isotope Probing Using ¹³C-Glucose

Published on: November 13, 2013

Extraction of nucleic acids followed by density gradient ultracentrifugation (e.g., in cesium chloride gradients).

Gradient fractionation to separate labeled and unlabeled DNA for subsequent molecular characterization.

Main Results:

Successful separation and purification of labeled DNA from environmental samples.
Demonstration of how DNA-SIP links microbial activity to specific carbon substrates.
Visual protocol aids in the accurate application of DNA-SIP methodology.

Conclusions:

DNA-SIP is a robust method for functional microbial community analysis.
The provided video protocol enhances the accessibility and reproducibility of DNA-SIP.
This technique is vital for understanding microbial metabolism in complex ecosystems.