An antibiotic selection marker for nematode transgenesis
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Updated: Jun 10, 2026

Visualizing Bacteria in Nematodes using Fluorescent Microscopy
Published on: October 19, 2012
Rosina Giordano-Santini1, Stuart Milstein, Nenad Svrzikapa
1Genome Regulation and Evolution, INSERM U869, Institut Européen de Chimie et Biologie, Pessac, France.
Abstract:
We have developed a nematode transformation vector carrying the bacterial neomycin resistance gene (NeoR) and shown that it could confer resistance to G-418 on both wild-type Caenorhabditis elegans and C. briggsae. This selection system allows hands-off maintenance and enrichment of transgenic worms carrying non-integrated transgenes on selective plates. We also show that this marker can be used for Mos1-mediated single-copy insertion in wild-type genetic backgrounds (MosSCI-biotic).
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Antibiotic Selection
Transduction

