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Nucleotide sequence of satellite I and II DNA from alpaca (Lama pacos) genome
A Sałuda-Gorgul1, J Jaworski, J Greger
1Department of Biochemistry I, Institute of Physiology and Biochemistry, Medical School of Lódź, Poland.
Abstract:
Agarose and polyacrylamide gel electrophoresis of a total alpaca (Lama pacos) DNA, digested with several restriction enzymes, revealed the presence of two tandemly organized repetitive DNA sequences, named Satellite I and Satellite II. Three Xhol-monomers from Satellite I DNA and two BspRI-monomers from Satellite II DNA were sequenced. As determined by dot hybridization analysis, the total alpaca DNA consists of 3.4% and 1.3% Satellites I and II, respectively. Computer search revealed no homology to any primate, rodent or mammalian sequences published in the Gen Bank Release 48.0 (February 1987) and the EMBL Bank Release 10 (December 1986).