Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ion Exchange01:17

Ion Exchange

Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or basic...
Capillary Exchange01:28

Capillary Exchange

The cardiovascular system's chief role is to disseminate gases, nutrients, waste, and other substances to the body's cells. Small molecules like gases, lipids, and lipid-soluble substances directly diffuse through capillary wall endothelial cell membranes. Glucose, amino acids, and ions, including sodium, potassium, calcium, and chloride, use transporters for facilitated diffusion via membrane-specific channels. Glucose, ions, and bigger molecules may also pass through intercellular clefts.
Synthetic Disvision of Polynomials01:28

Synthetic Disvision of Polynomials

Synthetic division is an efficient algorithmic approach for dividing a polynomial by a linear binomial of the form x - c, where c is a real number. This method is helpful due to its streamlined process, which avoids the more cumbersome steps involved in the traditional long division of polynomials. It simplifies computation and serves as a practical tool for evaluating polynomials and identifying their factors.To perform synthetic division, one begins by listing the coefficients of the...
¹H NMR Signal Multiplicity: Splitting Patterns01:13

¹H NMR Signal Multiplicity: Splitting Patterns

When protons A and X are coupled, their nuclear spin energy levels are slightly modified. This is because the energy required to excite proton A to a spin state parallel to proton X is slightly different from the energy required for it to become anti-parallel to spin X. Consequently, there are two possible excitation frequencies for A (A1 and A2), depending on the spin state of X, and vice versa. The mutual nature of coupling implies that the difference between frequencies A1 and A2, indicated...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule01:10

Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule

In the AX proton spin system, proton A can sense the two spin states of a coupled proton X, resulting in a doublet NMR signal with two peaks of equal (1:1) intensity. When proton A is coupled to two equivalent protons (AX2 spin system), the spin states of each X can be aligned with or against the external field, creating three possible scenarios. This results in a 1:2:1  triplet signal, where the central peak corresponds to the chemical shift of A and is twice as large or intense as the others.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Conformational dynamics as an intrinsic determinant of prion protein misfolding and neurotoxicity.

Neural regeneration research·2024
Same author

Intrinsic determinants of prion protein neurotoxicity in <i>Drosophila</i>: from sequence to (dys)function.

Frontiers in molecular neuroscience·2023
Same author

Y225A induces long-range conformational changes in human prion protein that are protective in Drosophila.

The Journal of biological chemistry·2023
Same author

Insight From Animals Resistant to Prion Diseases: Deciphering the Genotype - Morphotype - Phenotype Code for the Prion Protein.

Frontiers in cellular neuroscience·2020
Same author

Correction to "FRET Analysis of Ionic Strength Sensors in the Hofmeister Series of Salt Solutions Using Fluorescence Lifetime Measurements".

The journal of physical chemistry. B·2020
Same author

FRET Analysis of Ionic Strength Sensors in the Hofmeister Series of Salt Solutions Using Fluorescence Lifetime Measurements.

The journal of physical chemistry. B·2020

Related Experiment Video

Updated: Jun 10, 2026

Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry
11:37

Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry

Published on: November 29, 2013

On the Interfragment Exchange in the X-Pol Method.

Alessandro Cembran1, Peng Bao, Yingjie Wang

  • 1Department of Chemistry and Minnesota Supercomputing Institute University of Minnesota, Minneapolis, MN 55455.

Journal of Chemical Theory and Computation
|August 24, 2010
PubMed
Summary

This study introduces X-Pol-X, a model incorporating exchange repulsion into explicit polarization (X-Pol) calculations. The findings show exchange repulsion energies are accurately and additively calculated for water clusters.

More Related Videos

Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline
11:09

Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline

Published on: January 5, 2017

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
08:06

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Published on: February 23, 2017

Related Experiment Videos

Last Updated: Jun 10, 2026

Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry
11:37

Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry

Published on: November 29, 2013

Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline
11:09

Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline

Published on: January 5, 2017

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
08:06

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Published on: February 23, 2017

Area of Science:

  • Computational Chemistry
  • Quantum Chemistry
  • Molecular Modeling

Background:

  • The explicit polarization (X-Pol) model is a computational method for describing intermolecular interactions.
  • Accurately accounting for exchange repulsion is crucial for precise modeling of molecular systems.

Purpose of the Study:

  • To incorporate exchange repulsion terms into the X-Pol model, creating a new method termed X-Pol-X.
  • To evaluate the performance of X-Pol-X for calculating exchange repulsion energies in molecular clusters.

Main Methods:

  • Antisymmetrizing the X-Pol Hartree-product wave function to develop X-Pol-X.
  • Utilizing block-localized wave functions (BLW) formalism within Hartree-Fock theory.
  • Applying the neglect of interfragment differential overlap (NIDO) approximation for Fock matrix simplification.

Main Results:

  • The X-Pol-X method successfully incorporates exchange repulsion into the X-Pol model.
  • Calculations on water trimers demonstrate that exchange repulsion energies are adequately treated.
  • Exchange repulsion energies were found to be pairwise additive to a good approximation in the studied systems.

Conclusions:

  • X-Pol-X provides an accurate and efficient approach for including exchange repulsion in polarization calculations.
  • The method shows promise for studying hydrogen-bonding interactions and other systems where exchange repulsion is significant.
  • The NIDO approximation offers a computationally feasible simplification for Fock matrix calculations.