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Related Concept Videos

Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

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Related Experiment Video

Updated: May 10, 2026

Chromatin Interaction Analysis with Paired-End Tag Sequencing (ChIA-PET) for Mapping Chromatin Interactions and Understanding Transcription Regulation
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Chromatin Interaction Analysis with Paired-End Tag Sequencing (ChIA-PET) for Mapping Chromatin Interactions and Understanding Transcription Regulation

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Transcriptional regulation by CHIP/LDB complexes.

Revital Bronstein1, Liron Levkovitz, Nir Yosef

  • 1Department of Microbiology and Biotechnology, Tel Aviv University, Tel Aviv, Israel.

Plos Genetics
|August 24, 2010
PubMed
Summary
This summary is machine-generated.

Investigating transcription factor complexes in Drosophila revealed that mutations in SSDP proteins alter the balance of CHIP-AP and CHIP-PNR complexes, impacting gene regulation during development.

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Transcription factors form diverse complexes, posing challenges in understanding gene regulation.
  • The nuclear adaptor CHIP/LDB is a key developmental regulator forming tissue-specific transcription complexes.
  • SSDP proteins are crucial cofactors that protect CHIP/LDB complexes from degradation.

Purpose of the Study:

  • To investigate the downstream targets and regulatory roles of CHIP/LDB transcription complexes.
  • To explore the impact of SSDP protein levels on CHIP/LDB complex activity and gene expression.
  • To identify genes involved in Drosophila development that interact with CHIP/LDB complex components.

Main Methods:

  • Utilized Drosophila genetics, microarray analysis, and bioinformatics.
  • Employed SSDP mutations to identify CHIP/LDB downstream targets.
  • Conducted genetic screens and in vivo RNAi silencing to identify interacting genes and novel roles.

Main Results:

  • Identified 189 downstream targets of CHIP/LDB, enriched for APTEROUS (AP) and PANNIER (PNR) binding sites.
  • Discovered 28 wing development and 23 thoracic bristle development genes interacting with CHIP/LDB components.
  • Uncovered novel roles for xbp1 and Gs-alpha in early wing and bristle development.

Conclusions:

  • Loss of SSDP disrupts the balance between CHIP-AP and CHIP-PNR transcription complexes.
  • This imbalance leads to differential regulation of target genes, affecting developmental processes.
  • Understanding combinatorial transcription complex regulation is vital for developmental gene battery studies.