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Updated: Jun 10, 2026

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture
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Microplate-reader compatible perfusion microbioreactor array for modular tissue culture and cytotoxicity assays.

Yuan Wen1, Xudong Zhang, Shang-Tian Yang

  • 1William G. Lowrie Dept. of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH 43210, USA.

Biotechnology Progress
|August 24, 2010
PubMed
Summary

A novel perfusion microbioreactor array enables high-throughput cell culture and drug testing. This 3D model revealed significant drug resistance in colon cancer cells compared to traditional 2D assays.

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Area of Science:

  • Biomedical Engineering
  • Tissue Engineering
  • Cell Biology

Background:

  • Tissue engineering offers advanced in vitro models for cell-based assays.
  • Perfusion microbioreactor arrays facilitate parallel microscale tissue culture.
  • High-throughput data generation remains a challenge in microscale culture systems.

Purpose of the Study:

  • To develop a plate-reader compatible perfusion microbioreactor array for high-throughput cell proliferation and cytotoxicity assays.
  • To integrate low-cost fibrous scaffolds for modular tissue culture.
  • To evaluate drug response in a 3D cell culture model.

Main Methods:

  • Fabrication of a 4x4 array of perfusion microbioreactors using multilayer soft lithography.
  • Integration of nonwoven polyethylene terephthalate fibrous matrices as tissue culture scaffolds.

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  • Culture of human colon cancer HT-29 cells and assessment of proliferation and cytotoxicity.

    • Main Results:

    • Achieved high cell density (over 5 x 10^7 cells/mL) in the microbioreactors with continuous perfusion.
    • Successfully quantified cell proliferation and cytotoxicity over time.
    • Observed significant drug resistance of HT-29 cells to 5-FU in the 3D perfusion culture compared to 2D assays.

    Conclusions:

    • The developed microbioreactor array is suitable for high-throughput, time-series cell-based assays.
    • 3D perfusion culture models exhibit differential drug responses compared to conventional 2D assays.
    • This technology advances in vitro modeling for drug discovery and personalized medicine.