Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Noninvasive methods to monitor dynamic single-cell events.

Trends in cell biology·2026
Same author

Towards a quantitative theory for transmission X-ray microscopy.

Beilstein journal of nanotechnology·2025
Same author

Evolving Rel.

Nature immunology·2025
Same author

Whole genome sequencing of CRISPR/Cas9-engineered NF-κB reporter mice for validation and variant discovery.

Scientific data·2024
Same author

XL-DNase-Seq: Footprinting Analysis of Dynamic Transcription Factors.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Co-imaging of RelA and c-Rel reveals features of NF-κB signaling for ligand discrimination.

Cell reports·2024

Related Experiment Video

Updated: Jun 10, 2026

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations
10:55

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Published on: December 16, 2017

Live cell imaging and systems biology.

Myong-Hee Sung1, James G McNally

  • 1Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. sungm@mail.nih.gov

Wiley Interdisciplinary Reviews. Systems Biology and Medicine
|August 24, 2010
PubMed
Summary
This summary is machine-generated.

Live cell microscopy overcomes limitations of in vitro data for systems biology. This quantitative imaging approach captures in vivo biochemistry in single cells, advancing molecular network analysis.

More Related Videos

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
07:14

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

Multiplexed Live-Cell Imaging for Drug Responses in Patient-Derived Organoid Models of Cancer
09:13

Multiplexed Live-Cell Imaging for Drug Responses in Patient-Derived Organoid Models of Cancer

Published on: January 5, 2024

Related Experiment Videos

Last Updated: Jun 10, 2026

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations
10:55

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Published on: December 16, 2017

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
07:14

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

Multiplexed Live-Cell Imaging for Drug Responses in Patient-Derived Organoid Models of Cancer
09:13

Multiplexed Live-Cell Imaging for Drug Responses in Patient-Derived Organoid Models of Cancer

Published on: January 5, 2024

Area of Science:

  • Molecular biology
  • Systems biology
  • Biochemistry

Background:

  • Mathematical models of molecular networks often rely on in vitro data.
  • In vitro measurements may not fully represent the complexity and individuality of single living cells.
  • Limitations of in vitro data hinder accurate systems biology models.

Purpose of the Study:

  • To survey the capabilities of live cell microscopy for in vivo biochemistry.
  • To illustrate how live cell imaging can analyze molecular networks.
  • To advocate for the integration of quantitative live-cell imaging in systems biology.

Main Methods:

  • Live cell microscopy techniques.
  • Quantitative imaging approaches.
  • In vivo biochemical analysis.

Main Results:

  • Live cell microscopy offers a powerful alternative to in vitro measurements.
  • Various imaging approaches can be applied to study molecular networks in real-time.
  • This technology enables the capture of cellular complexity and individuality.

Conclusions:

  • Live cell microscopy is essential for overcoming the limitations of in vitro data.
  • Quantitative live-cell imaging is critical for the advancement of systems biology.
  • In vivo biochemistry through microscopy enhances the accuracy of molecular network models.