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Related Experiment Videos

Disk elution method for MICs and MBCs.

E Wilson1, D A Henry, J A Smith

  • 1Division of Medical Microbiology, Vancouver General Hospital, British Columbia, Canada.

Antimicrobial Agents and Chemotherapy
|November 1, 1990
PubMed
Summary
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A novel method using antimicrobial susceptibility testing disks for Minimum Inhibitory Concentration (MIC) determination is accurate and reproducible. This disk elution method offers a reliable alternative for clinical laboratories performing antimicrobial susceptibility testing.

Area of Science:

  • Clinical Microbiology
  • Antimicrobial Resistance
  • Diagnostic Test Development

Background:

  • Accurate antimicrobial susceptibility testing is crucial for guiding patient treatment and monitoring resistance.
  • Standard methods for determining Minimum Inhibitory Concentrations (MICs) can be resource-intensive.
  • Developing efficient and reliable MIC determination methods is an ongoing challenge in clinical laboratories.

Purpose of the Study:

  • To evaluate a novel Minimum Inhibitory Concentration (MIC) macrodilution broth method utilizing routine antimicrobial susceptibility testing disks.
  • To compare the accuracy and reproducibility of this disk elution method against the established National Committee for Clinical Laboratory Standards (NCCLS) M7A reference method.
  • To assess the utility of the disk elution method for routine use in clinical laboratories.

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Main Methods:

  • A macrodilution broth method was developed using antibiotics eluted from standard antimicrobial susceptibility testing disks.
  • The method was validated using American Type Culture Collection (ATCC) quality control strains and known laboratory isolates.
  • Antibiotic elution time was optimized to 1 hour, with concentrations confirmed using TDX and bioassay methods.
  • Minimum Inhibitory Concentrations (MICs) and Minimum Bactericidal Concentrations (MBCs) were determined and compared to the NCCLS M7A reference method over five test runs.

Main Results:

  • The disk elution method demonstrated high reproducibility, with 97% agreement for MICs compared to the reference method within +/- 1 dilution.
  • Minimum Bactericidal Concentration (MBC) determination showed 93% agreement with the reference method.
  • The method proved accurate and reproducible across multiple test runs using various antibiotics (gentamicin, piperacillin, oxacillin).

Conclusions:

  • The disk elution method using routine antimicrobial susceptibility testing disks is a reproducible and accurate system for determining MICs.
  • This method offers a reliable and potentially cost-effective alternative for clinical laboratories already performing disk susceptibility testing.
  • The validated disk elution technique can be readily implemented in laboratories to enhance antimicrobial susceptibility testing capabilities.