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Updated: Jun 10, 2026
The Analysis of Neurovascular Remodeling in Entorhino-hippocampal Organotypic Slice Cultures
Published on: October 23, 2014
[Expression of vascular endothelial growth factor and microvessel density in hippocampus of rats with aging]
Hai-qin Wu1, Bei Ren, Juan-juan Sha
1Department of Neurology, The Second Affiliated Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710004, China. whq60@sohu.com
Objective:
To investigate the expression of vascular endothelial growth factor (VEGF) and microvessel density (MVD) in hippocampus of rats with aging.
Methods:
Paraffin sections of brain tissue of rats at the age of 3, 18, 24, 30 months were stained by immunohistochemistry, the expression of VEGF and MVD was quantitatively analyzed.
Results:
Innunohistochemical staining showed that the VEGF-positive cells were mainly pyramidal neuron in hippocampus; the intensity of VEGF-positivity in neuron cells was decreased with the aging (P<0.05). The MVD in hippocampus was also decreased with the aging of rats (P<0.05).
Conclusion:
Increasing VEGF contents and improving blood circulation in brain tissue may prevent or treat vascular dementia and cerebrovascular diseases.
