Related Experiment Video
Updated: Jun 10, 2026
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
[Study on growth inhibition of implanted tumor in nude mice by antisense oligodeoxynucleotide targeting telomerase
Hongjun Wang1, Qiong Chen, Hongtao Yu
1Department of respiratory medicine of The Fifth People's Hospital, Zhengzhou 450000 China.
Background:
Many studies have proven that telomerase plays an important role during the occurrence and development of lung cancer, which has been an important target in gene therapy. The aim of this study is to explore the inhibitory effect of antisense oligodeoxynucleotides(ASODN) targeting human telomerase RNA on implanted tumor growth in nude mice.
Methods:
Implanted tumor models were Constructed in nude mice using human lung adenocarcinoma cell line A549. Eighteen Balb/C nude mice with implanted tumors were divided into three groups randomly: antisense oligodeoxynucleotide group(group ASODN), sense oligodeoxynucleotide group(group SODN), normal saline group (group NS), with 6 mice respectively. ASODs or SODNs mixed with Lipofectamine-2000 was injected into tumor directly every 24 h for fourteen days.
Results:
The inhibitory rates of tumor volume in Group ASODN and Group SODN were 43.94% and 6.91%, respectively. There was significant difference between the two groups(t =6.17, P<0.001). All the animals tolerated the drugs well and there was no nausea, vomiting and subcutaneous hemorrhage to occur during the therapy. The weight of the animals increased slightly at the end of experiment.
Conclusions:
The results of this study suggest that intratumoral injection of human telomerase ribonucleic acid(hTR) ASOND could effectively inhibit the growth of implanted tumor in nude mice.
Insights
Antisense oligodeoxynucleotides targeting human telomerase RNA effectively inhibited implanted lung cancer tumor growth in mice. This gene therapy approach shows promise for treating lung adenocarcinoma by targeting telomerase.
Area of Science:
- Oncology
- Molecular Biology
- Gene Therapy
Context:
- Telomerase is crucial in lung cancer development and progression.
- Telomerase is a validated target for anti-cancer gene therapy.
Purpose:
- To evaluate the efficacy of antisense oligodeoxynucleotides (ASODN) targeting human telomerase RNA (hTR) in inhibiting lung cancer tumor growth.
- To assess the safety and tolerability of ASODN therapy in a preclinical model.
Summary:
- Human lung adenocarcinoma cells (A549) were implanted in nude mice to create tumor models.
- Mice were treated with ASODN, sense oligodeoxynucleotides (SODN), or normal saline via direct intratumoral injection.
- ASODN treatment resulted in a significant 43.94% inhibition of tumor volume compared to SODN (6.91%) and control groups, with no observed adverse effects.
Impact:
- Intratumoral injection of hTR ASODN demonstrates significant potential as a therapeutic strategy against lung cancer.
- This study provides preclinical evidence supporting the development of telomerase-targeted gene therapies for lung adenocarcinoma.
Related Concept Videos
02:15Replicative Cell Senescence
02:15Experimental RNAi