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[Effect of 20(R) ginsenoside Rg3 on protein expression of lung cancer cell line.]
Ning An1, Wen Zhu, Zhihua Feng
1The State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
Background:
Lung cancer is the most dangerous threating tumor to human's health and life. Metastasis is not only the malignant characteristics of lung cancer, but also the chief cause of failure to cure the disease and of high mortality. Ginsenoside Rg3 has been proved to have obvious effect against tumor. The molecular mechanism of Rg3 against lung cancer cell line will be investigated by using two-dimensional gel electrophoresis in this paper.
Methods:
The IC50 of Rg3 against human high-metastatic large cell lung cancer cell line 95D was determined by MTT. Then 95D cells were treated with Rg3 at the concentration of 0.1*IC50 for 72 h. The total proteins of 95D cell line treated with Rg3 and not treated were separated and protein profiles were obtained by using immobilized PH gradient (IPG)-based two-dimensional gel electrophoresis. The differential expression proteins of 95D cell line treated with Rg3 and not treated were analyzed using image analysis software.15 of differentially expressed proteins were further identified using MALDI-TOF MS/MS analysis and LC-MS/MS analysis. Protein identification was performed by searching the protein database.
Results:
The IC50 of Rg3 against 95D cell line was 100 mug/mL. There were 27 differently expressed protein spots through analysis by Image Master Microsoft.15 proteins were identified using mass spectrometry. Chloride intracellular channel protein 1, Ubiquitin-Conjugating Enzyme E2-25 Kda only expressed in control; 14-3-3 protein teta, SKI-interacting protein only expressed in 95D cell line treated by Rg3; Annexin A2, profilin 2 isoform b were downregulated in 95D cell line treated by Rg3; 14-3-3 protein zeta, Eukaryotic translation initiation factor 4H were upregulated in 95D cell line treated by Rg3.
Conclusions:
A significantly different expression of proteins were found in 95D cell line treated with Rg3 and those not treated. Most of identified proteins have been reported to be associated with tumor metastasis. The identified proteins will provide the basis for searching potential biomarkers and the molecular mechanism of Rg3 against the metastasis of lung cancer cells.
Insights
Ginsenoside Rg3 significantly alters protein expression in lung cancer cells, revealing potential therapeutic targets. This study identifies key proteins involved in metastasis, offering insights into Rg3
Area of Science:
- Proteomics
- Molecular Biology
- Cancer Research
Context:
- Lung cancer metastasis is a primary cause of mortality.
- Ginsenoside Rg3 demonstrates anti-tumor properties.
- Understanding the molecular mechanisms of Rg3 is crucial for developing targeted therapies.
Purpose:
- To investigate the molecular mechanism of Ginsenoside Rg3 against lung cancer metastasis.
- To identify differentially expressed proteins in lung cancer cells treated with Rg3 using 2D gel electrophoresis and mass spectrometry.
Summary:
- Two-dimensional gel electrophoresis and mass spectrometry identified 15 differentially expressed proteins in 95D lung cancer cells treated with Ginsenoside Rg3.
- Key proteins such as Chloride intracellular channel protein 1, Ubiquitin-Conjugating Enzyme E2-25 Kda, 14-3-3 protein teta, and Annexin A2 showed altered expression.
- Most identified proteins are associated with tumor metastasis, providing insights into Rg3's anti-metastatic effects.
Impact:
- Identified proteins serve as potential biomarkers for lung cancer metastasis.
- Provides a basis for understanding the molecular mechanisms of Ginsenoside Rg3 in inhibiting lung cancer cell metastasis.
- May lead to the development of novel therapeutic strategies targeting lung cancer metastasis.