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Testing ocular irritancy in vitro with the silicon microphysiometer.

L H Bruner1, K R Miller, J C Owicki

  • 1The Procter & Gamble Company, Miami Valley Laboratories, Cincinnati, OH 45239, USA.

Toxicology in Vitro : an International Journal Published in Association with BIBRA
|August 25, 2010
PubMed
Summary
This summary is machine-generated.

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This study validates a silicon microphysiometer as a promising in vitro alternative for ocular irritancy testing. The instrument accurately correlates cell metabolism changes with in vivo rabbit eye test data for various consumer products.

Area of Science:

  • Toxicology
  • Biotechnology
  • Materials Science

Background:

  • Ocular irritancy testing traditionally relies on animal models, raising ethical and efficiency concerns.
  • Developing reliable in vitro alternatives is crucial for modern safety assessments.
  • The light-addressable potentiometric sensor offers a novel approach to non-invasive cellular analysis.

Purpose of the Study:

  • To evaluate the silicon microphysiometer as an in vitro alternative for assessing ocular irritancy potential.
  • To correlate the instrument's measurements with established in vivo rabbit eye test data.
  • To explore the potential of multiple endpoints for comprehensive ocular safety evaluations.

Main Methods:

  • Utilized a silicon microphysiometer employing a light-addressable potentiometric sensor.

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  • Measured cell metabolism by quantifying acid metabolite production from human epidermal keratinocytes.
  • Tested 17 liquid materials, including soaps, detergents, and shampoos, comparing results to historical in vivo rabbit eye test data.

    • Main Results:

    • Demonstrated a strong positive correlation (r = 0.86, P < 0.0001) between in vivo irritancy and the concentration causing a 50% decrease in cell acidification rate (MRD(50)).
    • Observed potential for additional endpoints, such as cell recovery and metabolic rate changes at sub-inhibitory concentrations.
    • Confirmed the non-invasive nature of the microphysiometer, allowing for assessment of cellular response and recovery.

    Conclusions:

    • The silicon microphysiometer shows significant potential as a reliable and ethical in vitro alternative for ocular safety testing.
    • The system's ease of use, strong in vivo-in vitro correlation, and multiple endpoints support its adoption.
    • Further research into additional endpoints may enhance its utility in ocular safety assessments.