Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 9, 2026

A Reversible Silicon Oil-Induced Ocular Hypertension Model in Mice
09:03

A Reversible Silicon Oil-Induced Ocular Hypertension Model in Mice

Published on: November 15, 2019

The silicon microphysiometer for testing ocular toxicity in vitro.

P Catroux1, A Rougier, K G Dossou

  • 1L'Oreal Basic Research Center, 1 avenue E. Schueller, 93600 Aulnay-Sous-Bois, France.

Toxicology in Vitro : an International Journal Published in Association with BIBRA
|August 25, 2010
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Algorithm for dermocosmetic use in the management of cutaneous side-effects associated with targeted therapy in oncology.

Journal of the European Academy of Dermatology and Venereology : JEADV·2013
Same author

[An investigational study of acne treatment in France].

Annales de dermatologie et de venereologie·2012
Same author

Interest of corrective makeup in the management of patients in dermatology.

Clinical, cosmetic and investigational dermatology·2012
Same author

Importance of treatment of skin xerosis in diabetes.

Journal of the European Academy of Dermatology and Venereology : JEADV·2011
Same author

Photoprotection in moisturizers and daily-care products.

Giornale italiano di dermatologia e venereologia : organo ufficiale, Societa italiana di dermatologia e sifilografia·2010
Same author

Measurement of inflammatory mediators produced by human keratinocytes in vitro: A predictive assessment of cutaneous irritation.

Toxicology in vitro : an international journal published in association with BIBRA·2010
Same journal

PM<sub>2.5</sub> exposure impairs mitochondrial function and myocardial contractility in cardiac organoids via the Sirt3/Mdh2 pathway.

Toxicology in vitro : an international journal published in association with BIBRA·2026
Same journal

Evaluation of an in-vitro test protocol using a three-dimensional human buccal oral mucosal model to assess the oral medical devices.

Toxicology in vitro : an international journal published in association with BIBRA·2026
Same journal

Descriptive oxidative and lipid-related fluorescence observations associated with Esketamine treatment in a high-concentration NMDA-exposed HT-22/C8-D1A co-culture model.

Toxicology in vitro : an international journal published in association with BIBRA·2026
Same journal

Integration of label-free electrochemical sensing, differential privacy deep learning, and blockchain for secure cytotoxicity monitoring.

Toxicology in vitro : an international journal published in association with BIBRA·2026
Same journal

Pulmonary permeability assessment using two human lung epithelial cell models in air-liquid interface cultures.

Toxicology in vitro : an international journal published in association with BIBRA·2026
Same journal

In vivo toxicity of some cyanide-containing compounds in zebrafish (Danio rerio) embryos.

Toxicology in vitro : an international journal published in association with BIBRA·2026
See all related articles

The silicon microphysiometer offers a reliable in vitro method for assessing ocular irritancy of water-soluble products. This cell-based assay shows high correlation with the in vivo Draize test, supporting its use as an alternative screening tool.

Area of Science:

  • Toxicology
  • Biomedical Engineering
  • Cell Biology

Background:

  • The Draize test is the standard for ocular irritancy assessment but involves animal testing.
  • There is a need for reliable in vitro alternatives to predict ocular irritation potential.
  • Silicon microphysiometry offers a novel approach to monitor cellular metabolic activity.

Purpose of the Study:

  • To evaluate the efficacy of the silicon microphysiometer for in vitro assessment of ocular irritancy.
  • To determine the correlation between silicon microphysiometer data and in vivo Draize test scores.
  • To assess the metabolic effects of various water-soluble ingredients and formulations on cultured cells.

Main Methods:

  • Utilized a silicon microphysiometer, a light-addressable potentiometric sensor, to monitor cellular metabolic rate.

More Related Videos

Development of an In Vitro Ocular Platform to Test Contact Lenses
08:28

Development of an In Vitro Ocular Platform to Test Contact Lenses

Published on: April 6, 2016

Related Experiment Videos

Last Updated: Jun 9, 2026

A Reversible Silicon Oil-Induced Ocular Hypertension Model in Mice
09:03

A Reversible Silicon Oil-Induced Ocular Hypertension Model in Mice

Published on: November 15, 2019

Development of an In Vitro Ocular Platform to Test Contact Lenses
08:28

Development of an In Vitro Ocular Platform to Test Contact Lenses

Published on: April 6, 2016

  • Exposed murine fibroblastic cells (L929 clone) to increasing concentrations of 53 products (surfactants and formulations).
  • Determined the MRD(50) (Metabolic Rate Decrease at 50%) and correlated it with historical Maximal Average Draize Scores (MAS).

    • Main Results:

    • The silicon microphysiometer effectively detected metabolic changes in cells exposed to test materials.
    • MRD(50) values varied widely across tested concentrations, indicating a broad range of sensitivities.
    • High linear (Pearson, r=0.91) and rank (Spearman, r=0.89) correlations were found between in vitro MRD(50) and in vivo MAS data.

    Conclusions:

    • The silicon microphysiometer method demonstrates a strong correlation with the Draize test for water-soluble substances.
    • This in vitro technique serves as a viable screening tool for predicting ocular irritation potential.
    • The study supports the adoption of this method as a cruelty-free alternative in product safety testing.