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Related Experiment Video

Updated: Jun 9, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Fourier-transform holographic microscope.

W S Haddad, D Cullen, J C Solem

    Applied Optics
    |August 25, 2010
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a novel holographic microscope achieving near-diffraction-limit resolution. The innovative design utilizes glycerol as a lens for superior 3D imaging, minimizing specimen damage.

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    Last Updated: Jun 9, 2026

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
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    Published on: July 5, 2016

    Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)
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    Published on: November 1, 2017

    Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
    10:16

    Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

    Published on: February 8, 2014

    Area of Science:

    • Optics and Photonics
    • Microscopy
    • Biophysics

    Background:

    • Traditional microscopy techniques face limitations in achieving high spatial resolution and minimizing photodamage.
    • Holographic microscopy offers potential for advanced 3D imaging but requires sophisticated illumination and reconstruction methods.

    Purpose of the Study:

    • To develop and characterize a holographic microscope with near-diffraction-limit spatial resolution.
    • To demonstrate the capability of collecting 3D information in a single light pulse and recording 3D motion pictures.
    • To explore the applicability of the conceptual design across a broad range of wavelengths, including X-ray.

    Main Methods:

    • Utilized a glycerol droplet as a lens for spherically diverging reference illumination in Fourier-transform holography.
    • Measured transverse resolution (1.4 microm at 514.5 nm) using a knife-edge test in dark-field illumination.
    • Achieved longitudinal resolution via numerical optical sectioning from recorded holograms.

    Main Results:

    • Demonstrated a transverse spatial resolution approaching the diffraction limit.
    • Successfully reconstructed 3D information from biological specimens using the developed method.
    • Showcased the ability to capture 3D data in a single light pulse and record 3D motion pictures.

    Conclusions:

    • The developed holographic microscope offers high-resolution 3D imaging with minimal specimen damage.
    • The instrument's design is versatile, applicable to various wavelengths and potentially extendable to X-ray microscopy.
    • This technology enables advanced biological imaging and dynamic 3D motion studies.