Related Experiment Videos
[Separation using RP-HPLC of non-human globin in the study of hemoglobin polymorphism]
1Istituto di Fisiologia Generale e Chimica Biologica, Università di Sassari.
Bollettino Della Societa Italiana Di Biologia Sperimentale
|September 1, 1990
Abstract:
The successful extension of the reversed-phase HPLC methodology to the study of non-human globin chains is shown. A large-pore C4 column, and simple acetonitrile in aqueous TFA gradients, were used to completely separate and quantitate globin chains from caprines, swines, rodents, and birds. Results indicate a large hemoglobin polymorphism in some species.