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Related Concept Videos

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 9, 2026

Femtosecond Laser Filaments for Use in Sub-Diffraction-Limited Imaging and Remote Sensing
06:16

Femtosecond Laser Filaments for Use in Sub-Diffraction-Limited Imaging and Remote Sensing

Published on: April 25, 2019

Imaging with femtosecond pulses.

C Yan, J C Diels

    Applied Optics
    |August 25, 2010
    PubMed
    Summary

    This study introduces an all-optical 3D imaging system. It uses direct upconversion and femtosecond gating for micrometer depth resolution and reduced scattering.

    Area of Science:

    • Optics and Photonics
    • Biomedical Imaging
    • Microscopy

    Background:

    • Traditional 3D imaging techniques often face limitations in resolution and scattering.
    • Optical methods are crucial for non-invasive visualization in various scientific fields.

    Purpose of the Study:

    • To describe a novel all-optical 3D imaging system.
    • To achieve high depth resolution and effective discrimination against scattered light.

    Main Methods:

    • Development of an all-optical system utilizing direct upconversion.
    • Implementation of femtosecond gating for signal processing.
    • Acquisition of depth-resolved images.

    Main Results:

    • Demonstration of micrometer-level depth resolution.

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    Last Updated: Jun 9, 2026

    Femtosecond Laser Filaments for Use in Sub-Diffraction-Limited Imaging and Remote Sensing
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    Published on: April 25, 2019

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  • Successful discrimination of images from scattered light.
  • All-optical 3D imaging capability achieved.
  • Conclusions:

    • The described system offers a promising approach for advanced 3D imaging.
    • Femtosecond gating is effective for enhancing image quality and depth penetration.
    • This technology has potential applications in fields requiring high-resolution 3D visualization.