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Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
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The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA (thiogalactoside...
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Cell Signaling in Plants01:25

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Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
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Constitutive and Regulated Gene Expression

Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Related Experiment Video

Updated: Jun 9, 2026

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
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Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

Inducible gene expression systems for plants.

Lorenzo Borghi1

  • 1Department of Biology, Swiss Federal Institute of Technology (ETH), Zurich, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)
|August 25, 2010
PubMed
Summary

Researchers can control transgene expression in plants using five inducible systems. These methods offer precise temporal and spatial gene regulation for studying essential genes and plant development.

Area of Science:

  • Plant Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Inducible systems enable controlled transgene expression in plants.
  • These systems are crucial for studying gene function, especially for essential genes.
  • Precise temporal and spatial control is vital for analyzing gene roles without disrupting overall plant development.

Purpose of the Study:

  • To present protocols for five distinct inducible plant gene expression systems.
  • To highlight the utility of these systems for precise genetic manipulation in plants.
  • To facilitate research requiring controlled gene expression in specific plant tissues or developmental stages.

Main Methods:

  • Detailed protocols for AlcR/AlcA (ethanol-inducible) system.
  • Protocols for dexamethasone-inducible systems: GR fusions, GVG, and pOp/LhGR.

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  • Protocols for XVE/OlexA (beta-estradiol-inducible) and heat shock induction systems.

    • Main Results:

    • Demonstration of five effective inducible systems for transgene expression.
    • Successful application in key plant species like tobacco, rice, Arabidopsis, tomato, and maize.
    • Enabling precise control over gene expression in specific plant tissues and developmental stages.

    Conclusions:

    • The presented inducible systems provide powerful tools for plant genetic research.
    • These methods allow for the study of essential genes and complex biological processes.
    • Researchers gain enhanced control over gene expression for advanced plant biotechnology applications.