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Updated: Jun 9, 2026

Immunoblot Analysis
Published on: June 20, 2008
Co-immunoprecipitation and protein blots
Erika Isono1, Claus Schwechheimer
1Department of Plant Systems Biology, Technical University Munich-Weihenstephan, Munich, Germany.
Abstract:
Knowledge about the identity of the interacting partners is important for the understanding of the function and the cellular activity of a given protein. Here we describe co-immunoprecipitation and pull-down as methods that are widely used for the identification and characterization of protein-protein interactions. These methods are well suited to find or confirm the interaction among multiple proteins, given the availability of a specific antibody for or a tagged version of the protein of interest.
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