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Related Concept Videos

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

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Related Experiment Video

Updated: Jun 9, 2026

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo
12:36

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo

Published on: January 14, 2016

Plant chromatin immunoprecipitation.

Corina B R Villar1, Claudia Köhler

  • 1Department of Biology, Swiss Federal Institute of Technology (ETH), Zurich, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)
|August 25, 2010
PubMed
Summary

This study details a chromatin immunoprecipitation (ChIP) protocol for Arabidopsis development. The method enables mapping of proteins and modifications to specific genomic regions, crucial for understanding gene expression.

Area of Science:

  • Developmental biology
  • Molecular biology
  • Genetics

Background:

  • Multicellular organism development relies on precise gene expression.
  • Chromatin structure and dynamics are critical regulators of transcription.
  • Chromatin immunoprecipitation (ChIP) is a key technique for mapping genomic elements.

Purpose of the Study:

  • To describe a robust chromatin immunoprecipitation (ChIP) protocol optimized for Arabidopsis.
  • To provide guidance on essential controls and data analysis for reliable ChIP results in plants.

Main Methods:

  • Adaptation of the chromatin immunoprecipitation (ChIP) technique for Arabidopsis samples.
  • Implementation of rigorous positive and negative controls.
  • Detailed discussion of data analysis methodologies for ChIP experiments.

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Chromatin Immunoprecipitation from Human Embryonic Stem Cells

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Related Experiment Videos

Last Updated: Jun 9, 2026

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo
12:36

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo

Published on: January 14, 2016

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass
14:29

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Published on: May 1, 2013

Chromatin Immunoprecipitation from Human Embryonic Stem Cells
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Chromatin Immunoprecipitation from Human Embryonic Stem Cells

Published on: July 22, 2008

Main Results:

  • A validated ChIP protocol for Arabidopsis material.
  • Demonstration of ChIP's utility in studying gene regulation during plant development.
  • Establishment of best practices for experimental design and analysis.

Conclusions:

  • The described ChIP protocol facilitates detailed investigation of chromatin dynamics in Arabidopsis.
  • Reliable ChIP data is essential for understanding gene regulation in developmental biology.
  • This protocol serves as a valuable resource for plant molecular biologists.