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Updated: Jun 9, 2026

A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation
Published on: July 6, 2017
Immunocytological analysis of chromatin in isolated nuclei
Penka Pavlova1, Federico Tessadori, Hans J de Jong
1Laboratory of Genetics, Wageningen University, Wageningen, Netherlands.
Abstract:
All cells in a multicellular organism have the same genetic constitution, yet their appearance and function may differ enormously, due to differences in the nuclear program. Central in the establishment of this cell diversity are epigenetic marks, which are largely based on covalent modifications of histones and methylated cytosine residues in the DNA sequence. The study of these epigenetic factors in individual cells requires the microscopic visualization of chromatin components. Here we describe a number of protocols to study chromatin in isolated nuclei.
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