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False negative results in high viremia parvovirus B19-samples tested with real-time PCR

Piotr Grabarczyk1, Aleksandra Kalińska, Ewa Sulkowska

  • 1Department of Immunohaematology, Institute of Haematology and Transfusion Medicine, Warsaw, Poland. pgrabarczyk@ihit.waw.pl

Polish Journal of Microbiology
|August 26, 2010
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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...