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Related Experiment Video

Updated: Jun 9, 2026

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
08:43

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

Developing a novel fiber optic fluorescence device for multiplexed high-throughput cytotoxic screening.

Dennis Lee1, Stephen Barnes

  • 1mAbGen LLC., R&D Department, 1250 Wood Branch Park Dr, Suite 625, Houston, TX 77079, USA. dlee@mabgenllc.com

Journal of Experimental Therapeutics & Oncology
|August 26, 2010
PubMed
Summary

The Ab-Sniffer, a new fiber optic fluorescence device, enables faster drug discovery by screening potential compounds using whole cells. This high-throughput cytotoxicity screening method improves efficiency in pharmaceutical research.

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Last Updated: Jun 9, 2026

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Area of Science:

  • Biotechnology
  • Pharmacology
  • Cell Biology

Background:

  • The drug discovery process is evolving with new technologies, necessitating faster methods.
  • High-throughput screening (HTS) is crucial for reducing drug discovery timelines.
  • Cell-based assays are vital for functional drug screening.

Purpose of the Study:

  • To introduce the Ab-Sniffer, a novel fiber optic fluorescence device for high-throughput cytotoxicity screening.
  • To demonstrate the Ab-Sniffer's utility for functional screening of potential drug compounds.
  • To present the developmental concept and early-stage testing of the Ab-Sniffer platform.

Main Methods:

  • Development of a fiber optic fluorescence device (Ab-Sniffer).
  • Utilizing immobilized whole cells with functionalized microspheres (biotin, streptavidin, Concanavalin A).
  • Adaptation of a cytotoxicity protocol using lymphoma cells and rituximab for validation.

Main Results:

  • Demonstrated the Ab-Sniffer's capability for functional screening of drug compounds.
  • Successfully employed an immobilized whole cell approach for cytotoxicity screening.
  • Validated the device using a known cytotoxicity assay with lymphoma cells.

Conclusions:

  • The Ab-Sniffer offers a novel platform for high-throughput cytotoxicity screening.
  • This method enables functional screening of drug compounds, moving beyond binding assays.
  • The platform is adaptable to various fluorescence-based assays and multi-target cell screening.