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Related Experiment Video

Updated: Jun 9, 2026

Advanced Glycation End-Products Sensitize Human Sensory-Like Neuron Cells to Capsaicin-Induced Calcium Influx
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Advanced glycation end products (AGEs) activate mast cells.

E Sick1, S Brehin, P André

  • 1Université de Strasbourg, Faculté de Pharmacie, Illkirch, France. emilie.sick@pharma.u-strasbg.fr

British Journal of Pharmacology
|August 26, 2010
PubMed
Summary

Advanced glycation endproducts (AGEs) activate mast cells via the RAGE receptor, triggering histamine release and reactive oxygen species (ROS) production. This interaction may fuel chronic inflammation and disease progression.

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Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice
09:07

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice

Published on: May 27, 2015

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Advanced glycation endproducts (AGEs) are protein modifications linked to aging and diseases like diabetes and cardiovascular conditions.
  • Mast cells are key players in inflammation and chronic disease progression.
  • The interaction between AGEs and mast cells is not fully understood.

Purpose of the Study:

  • To investigate the interaction between AGEs and mast cells.
  • To determine the role of the receptor for AGEs (RAGE) in this interaction.
  • To assess the downstream signaling events initiated by AGEs in mast cells.

Main Methods:

  • Histamine secretion assays from AGEs-stimulated mast cells.
  • RAGE expression analysis using PCR and immunostaining.
  • Inhibition studies using RAGE antagonists and signaling pathway inhibitors.
  • Measurement of reactive oxygen species (ROS) and cytokine production.

Main Results:

  • AGEs dose-dependently induced mast cell exocytosis within seconds.
  • RAGE mRNA was detected, and RAGE was present on mast cell surfaces.
  • AGE-induced exocytosis was blocked by anti-RAGE antibodies and heparin.
  • AGEs rapidly stimulated ROS production, but cytokine secretion remained unchanged after 6 hours.
  • AGE-RAGE signaling involved G(i)-proteins and intracellular calcium increases.

Conclusions:

  • AGEs activate mast cells, contributing to inflammation.
  • AGEs-RAGE interaction may create a cycle of ROS generation and AGE formation.
  • This pathway could exacerbate low-grade inflammation in chronic diseases.