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Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Related Experiment Video

Updated: Jun 9, 2026

Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays for High-Throughput Large-Scale Sample Inspection

Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays for High-Throughput Large-Scale Sample Inspection

Published on: June 13, 2023

High throughput cell nanomechanics with mechanical imaging interferometry.

Jason Reed¹, Matthew Frank, Joshua J Troke

¹Department of Chemistry and Biochemistry, UCLA, 607 Charles Young Drive East, Los Angeles, CA 90095, USA.

|August 26, 2010

Summary

This summary is machine-generated.

This study introduces a novel nanotechnology for high-throughput measurement of cell nanomechanical properties. The technique quantifies viscoelasticity in large cell arrays with high resolution and sensitivity.

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Last Updated: Jun 9, 2026

Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays for High-Throughput Large-Scale Sample Inspection

Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays for High-Throughput Large-Scale Sample Inspection

Published on: June 13, 2023

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

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Published on: June 27, 2013

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements

Published on: December 2, 2022

Area of Science:

Biophysics
Cell Biology
Nanotechnology

Background:

Understanding cellular mechanical properties is crucial for cell biology and disease research.
Existing methods often lack the throughput or resolution to study large cell populations.

Purpose of the Study:

To develop and validate a novel nanotechnology for high-throughput, parallel measurement of live cell nanomechanical properties.
To quantify the local viscoelastic properties of fibroblasts using a wide range of forces and high spatial resolution.

Main Methods:

Utilized imaging interferometry combined with reflective magnetic probes attached to cells.
Applied forces ranging from 20 pN to 20 nN with spatial resolution under 20 nm.
Measured nanomechanical properties of NIH 3T3 and HEK 293T fibroblasts, including responses to actin depolymerizing drugs.

Main Results:

Successfully measured dynamic nanomechanical properties of hundreds of cells in parallel.
Demonstrated a mechanical dynamic range from several Pascals to approximately 200 kilopascals.
Validated the sensitivity and scalability of the mechanical imaging interferometry technique.

Conclusions:

Mechanical imaging interferometry is a sensitive and scalable technology for nanomechanical property assessment.
This approach enables high-throughput analysis of live cells in fluidic environments.
The technology has potential applications in cell biology, drug screening, and disease diagnostics.