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Updated: May 18, 2026

11:54
Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
Published on: March 23, 2020
Synthesis of K48-linked diubiquitin using dual native chemical ligation at lysine
Renliang Yang1, Kalyan Kumar Pasunooti, Fupeng Li
1Division of Chemical Biology and Biotechnology, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551.
Abstract:
The dual native chemical ligation at lysine strategy was revised by replacing the acid-labile Cbz protecting group with photolabile NVOC at the 4-mercaptolysine side chain. The optimized strategy was subsequently applied to the synthesis of K48-linked diubiquitin.

