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Class IIa bacteriocin resistance in Enterococcus faecalis V583: the mannose PTS operon mediates global

Mona Opsata1, Ingolf F Nes, Helge Holo

  • 1Laboratory of Microbial Gene Technology and Food Microbiology, Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Norway. mona.opsata@umb.no

BMC Microbiology
|August 27, 2010
PubMed
Abstract

Insights

Researchers identified specific gene expression changes in Enterococcus faecalis mutants resistant to pediocin PA-1. This resistance is linked to the mannose phosphotransferase system (PTS), impacting carbon catabolite control.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genomics

Background:

  • Pediocin PA-1, a class IIa bacteriocin, shows promise as a food preservative and for combating Gram-negative pathogens like Enterococcus faecalis and Listeria monocytogenes.
  • Understanding bacteriocin resistance mechanisms is crucial for optimizing antimicrobial applications.
  • This study investigates pediocin PA-1 resistance in E. faecalis V583 using transcriptomic analysis.

Purpose of the Study:

  • To identify genetic factors contributing to pediocin PA-1 resistance in Enterococcus faecalis.
  • To elucidate the role of specific gene expression changes in bacteriocin resistance phenotypes.
  • To explore the connection between bacteriocin resistance and carbon catabolite control.

Main Methods:

  • Isolation and characterization of pediocin PA-1 resistant mutants of E. faecalis V583.
  • Whole-genome microarray analysis to compare gene expression profiles between resistant mutants and wild-type strains.
  • Transcriptomic analysis of a mptD-inactivated mutant to confirm the role of the mannose phosphotransferase system (PTS).

Main Results:

  • Approximately 200 genes exhibited significantly altered transcription in resistant mutants compared to the wild type.
  • Glycolytic genes were downregulated, while most other differentially expressed genes were upregulated, suggesting relief from glucose repression.
  • Bacteriocin resistance was associated with reduced expression of the mpt operon (mannose-specific PTS), indicating the PTS's involvement in its own transcriptional regulation.

Conclusions:

  • The mannose phosphotransferase system (PTS) plays a critical role in sensitivity to class IIa bacteriocins.
  • The study demonstrates the involvement of the mannose PTS in global carbon catabolite control.
  • These findings provide insights into bacteriocin resistance mechanisms and their implications for antimicrobial applications.

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