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Evaluation of a gold-silver staining method for detection and identification of Candida species by light microscopy
J C Cailliez1, A Boudrissa, D W Mackenzie
1Institut National de la Santé et de la Recherche Médicale, Unité 42 de Biologie et de Biochimie Parasitaires et Fongiques, Villeneuve, France.
Abstract:
A gold-silver staining procedure was evaluated for detection of Candida species of medical importance. Probes were prepared by coupling lectins or antibodies (polyclonal and monoclonal) directly or indirectly to colloidal gold particles. Structures reacting to these probes were specifically revealed by light microscopy in cells present in infected kidney tissue sections or in isolated yeast cells on glass slides. Definition, contrast and sensitivity were of a high order. Preliminary data showed that it was possible, using discriminating dilutions, to identify cells from different species of the genus Candida, grown in vitro, according to their ability to stain with polyclonal monospecific antisera. The advantages of gold-silver staining compared with other staining procedures currently used in routine mycological laboratories are its sensitivity, good definition, ease and rapidity, and long conservation of reaction. It is suggested that the procedure has applications for research and identification of yeasts in clinical samples.