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Serological evidence of human klassevirus infection

Alexander L Greninger1, Lori Holtz, Gagandeep Kang

  • 1Departments of Molecular Microbiology and Pathology and Immunology, Howard Hughes Medical Institute,Washington University, St. Louis, MO 63110, USA.

Clinical and Vaccine Immunology : CVI
|August 27, 2010
PubMed

Insights

Klassevirus infection occurs in humans, as shown by seroconversion in pediatric diarrhea patients. This new picornavirus genus has a 6.8% seroprevalence in St. Louis, indicating widespread circulation.

Area of Science:

  • Virology
  • Infectious Diseases
  • Pediatric Gastroenterology

Background:

  • Klassevirus, a proposed new picornavirus genus, has been linked to pediatric diarrhea.
  • Understanding the prevalence and human infection dynamics of Klassevirus is crucial for public health.

Purpose of the Study:

  • To develop and validate a serological assay for detecting Klassevirus infection in humans.
  • To investigate evidence of Klassevirus infection and replication in individuals with pediatric diarrhea.
  • To determine the seroprevalence of Klassevirus in a defined human population.

Main Methods:

  • Utilized recombinant klassevirus 3C protease as a capture antigen in an indirect enzyme-linked immunosorbent assay (ELISA).
  • Tested serum samples from individuals positive for Klassevirus by reverse transcription polymerase chain reaction (RT-PCR).
  • Screened a cohort of 353 individuals from St. Louis hospitals using an age-banded serological study.

Main Results:

  • Four out of six Klassevirus RT-PCR-positive individuals showed seroconversion against the 3C protease.
  • This seroconversion suggests active Klassevirus infection and replication in humans.
  • A seroprevalence of 6.8% was identified in the screened population.

Conclusions:

  • The study provides serological evidence supporting Klassevirus infection in humans.
  • The findings indicate that Klassevirus is circulating in the human population.
  • Further research is warranted to understand the clinical significance and transmission of Klassevirus.

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